Biohydrogen Production from Agro-industrial Wastes Using Clostridium beijerinckii and Isolated Bacteria as Inoculum

The objective of this work was to evaluate the potential biohydrogen production in agro-industrial residues, which were soft drink wastewater (SDW), corn steep liquor (CSL), cheese whey (CW), and expired Guaraná drink, using one model strain Clostridium beijerinckii ATCC 8260 newly isolated butyricum DEBB-B348. residues characterized, all them contained significant concentrations carbohydrates such as glucose, fructose, lactose, maltose, case CSL CW they also amino acids. After performing subsequent experimental designs, factors concentration, fermentation time for C. beijerinckii, concentration (p ? 0.05), with R2 0.950 0.895, respectively. maximum hydrogen volume 18.5 ± 1.68 mL 27.4 1.84 each strain, It is noteworthy high yield produced (235 H2/g COD removed) (310 removed). noted that and/or CW-based culture media do not need be supplemented cysteine-HCl. These results indicate a process utilizing less expensive substrates.